RNA-sequencing analysis reveals key genes behind eggplant peel variation

Horticulture Research published research titled “RNA-sequencing analysis reveals novel genes involved in the different peel color formation in eggplant.”

Firstly, the expression patterns of anthocyanin biosynthesis genes in eggplants of different colors and developmental stages were explored, and it was found that anthocyanin biosynthesis genes were more expressed in purple eggplant varieties, especially in the rapid growth stage.

RNA seq analysis was performed on the G-stage eggplant skin to identify differentially expressed genes (DEGs) related to anthocyanin and flavonoid biosynthesis. Particularly, specific genes, including SmMYB113 and WRKY44, were up-regulated in cultivars with higher delphinidin/flavonoid ratios, suggesting their involvement in color variation. Furthermore, the study uncovered 27 novel genes potentially linked to color differences and 32 novel genes within the SmMYB113-regulated anthocyanin biosynthesis network.

Notably, five genes (SmCytb5, SmGST, SmMATE, SmASAT3, and SmF3’5’M) were identified as crucial for both color variation and anthocyanin accumulation. The expression of these five genes was directly activated by SmMYB113 through yeast one-hybrid, electrophoretic mobility shift assay, and dual luciferase experiments.

Overall, the research not only elucidates the transcriptional basis of color variation in eggplant peels but also highlights SmMYB113’s central role in regulating anthocyanin biosynthesis, offering insights for future genetic and breeding strategies to enhance fruit coloration.

More information:
Jing Li et al, RNA-sequencing analysis reveals novel genes involved in the different peel color formation in eggplant, Horticulture Research (2023). DOI: 10.1093/hr/uhad181