Photodynamic action weakens resistance to antibiotics in bacteria that attack airways

The research group analyzed patient samples containing Staphylococcus aureus, a bacterium that causes a range of diseases from skin infections to pneumonia. Credit: Janice Haney Carr/CDC PHIL The development of antibiotics to combat multidrug-resistant bacteria, especially those that infect the airways, has been increasingly tricky, and some scientists have opted to try to weaken the

Liked this post? Share with others!

Hexbyte Glen Cove

The research group analyzed patient samples containing Staphylococcus aureus, a bacterium that causes a range of diseases from skin infections to pneumonia. Credit: Janice Haney Carr/CDC PHIL

The development of antibiotics to combat multidrug-resistant bacteria, especially those that infect the airways, has been increasingly tricky, and some scientists have opted to try to weaken the bacteria so that the available therapeutic substances are made more effective.

This approach is promising, as shown by an article published in the journal Proceedings of the National Academy of Sciences (PNAS), which concludes that photodynamic inactivation (PDI) showed a novel capacity to modify bacterial sensitivity to antibiotics according to dosage, reducing the resistance and persistence of both standard and clinical strains.

The lead author is Vanderlei Salvador Bagnato, a physicist and materials engineer at the University of São Paulo’s São Carlos Institute of Physics (IFSC-USP) in Brazil.

The study focused on Staphylococcus aureus, a bacterium that causes a range of diseases from skin infections to pneumonia, investigating the effects of photodynamic action on resistant bacteria collected from patients and bacterial cells with laboratory-induced resistance. The results showed that five cycles of PDI were sufficient to break their resistance.

In PDI, a dye called a photosensitizer is energized by absorbing visible light to form that can oxidize and destroy microorganisms or weaken their antibiotic resistance.

The researchers used 10 μM curcumin as the photosensitizer and worked with three antibiotics—amoxicillin, erythromycin, and gentamicin. After the five cycles of PDI, they found that S. aureus was most susceptible to gentamicin, although the other two antibiotics also proved effective against the bacteria after PDI.

“We discovered that PDI doesn’t always destroy the bacteria, but it does destroy part of the mechanisms they use to become drug-resistant. This led to the idea of trying an oxidative shock to make them susceptible to antibiotics,” Bagnato told Agência FAPESP. Bagnato is principal investigator for the Optics and Photonics Research Center (CePOF).

The first author of the article is Jennifer Soares, a researcher at IFSC-USP and CePOF. As a former Ph.D. candidate, she studied under Bagnato and co-author Kate Cristina Blanco, also a professor at IFSC-USP and a member of CePOF.

Hexbyte Glen Cove Multidrug-resistant bacteria

The World Health Organization (WHO) has prioritized antimicrobial resistance (AMR) as one of the top 10 global public health threats facing humanity. AMR is a process that occurs as bacteria, viruses, fungi, and parasites change over time and no longer respond to antibiotics and antivirals, for example.

The WHO estimates that some 1.2 million deaths are caused directly by AMR every year, and almost 5 million are indirectly associated with it. AMR could cost the global economy USD 100 trillion by 2050 if no action is taken.

According to a report issued last year by the WHO, out of every 100 patients in acute-care hospitals, seven patients in and 15 patients in low- and will acquire at least one health care-associated infection (HAI) during their hospital stay. On average, 1 in every 10 affected patients will die from their HAI. “Deaths are increased two to threefold when infections are resistant to antimicrobials,” the report adds.

According to the PNAS article, the chances of approval of new antibiotics by the US Food and Drug Administration (FDA) for clinical trials in humans is 6 out of 10, and the probability that those approved will be a new antibiotic class is only 25%, which “implies a low probability of solving the bacterial resistance problem since most new antimicrobials tend to derive from existing classes.”

Bagnato’s research has focused for several years on drug-resistant pneumonia, one of the drug-resistant infections that most frequently cause deaths in intensive care units. “We’re about to publish an article describing a technique applied directly in the lungs. The patient inhales an inductive molecule, and we do extracorporeal infrared illumination to weaken the microorganism’s resistance as part of a strategy to combat pneumonia, for example,” he said.

More information:
Jennifer M. Soares et al, Recovering the susceptibility of antibiotic-resistant bacteria using photooxidative damage, Proceedings of the National Academy of Sciences (2023). DOI: 10.1073/pnas.2311667120

Citation:
Photodynamic action weakens resistance to antibiotics in bacteria that attack airways (2023, December 18)
retrieved 18 December 2023
from https://phys.org/news/2023-12-photodynamic-action-weakens-resistance-

% %item_read_more_button%% Hexbyte Glen Cove Educational Blog Repost With Backlinks — #metaverse #vr #ar #wordpress

Subscribe to our newsletter

Collect visitor’s submissions and store it directly in your Elementor account, or integrate your favorite marketing & CRM tools.

Do you want to boost your business today?

This is your chance to invite visitors to contact you. Tell them you’ll be happy to answer all their questions as soon as possible.

Learn how we helped 100 top brands gain success