Discovery in mosquitoes could lead to new strategy against dengue fever and other mosquito-borne vectors

Generation of Ago2 knockout Ae. aegypti lines and the effect of Ago2 disruption on arbovirus infection and transmission. a flowchart of experiment design. b gene structure of Ago2, predicted functional domains, and guide RNA (gRNA) design for CRISPR/Cas9. The table shows data from generating Ago2 knockout lines. c Ago2 amplification in Ago2 knockout lines. P

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